The lightcycler 480 ii is a realtime pcr cycler for mid to high sample throughput. Lightcycler technology is the most innovative and rapid possibility for carrying out and simultaneously evaluating pcr experiments. Mar 31, 2020 i am using lightcycler 96 for absolute quantification. The versatile, platebased lightcycler 96 system helps you achieve fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and snp analysis compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis.
Contents of the workstation the lightcycler components are listed in the following table. The exchanged block cycler unit is automatically detected and identified by the system, and experiments can be pursued without time. The lightcycler instrument of roche molecular biochemicals, mannheim, ge rmany, is a thermocycler for the rapid analysis of pcr applications. A low target concentration 100 copies of a given target sequence 442 bp was amplified using a fast pcr protocol 20 l reaction volume, hydrolysis probe format. Primepcr assays, panels, and controls for realtime pcr. Use of the roche lightcycler instrument in a realtime pcr. The machine is good, consistent, has never needed maintenance even. National scientific offers an extensive range of tubes, strips and multiwell plates for the pcr process. The lightcycler 480 instrument is an authorized thermal cycler.
Lightcycler 480 thermal block cycler unit 96384well aluminum. How to generate qpcr standard curve in lightcycler 96. Super capabilities are now within your reach genequantification. The lightcycler 96 system embodies roche expertise over the last decade in developing and providing highperformance qpcr systems that enable research breakthroughs. But i didnt manage to use it, because the pcr apparatus is a lightcycler 480. Primer pairs are lyophilized in the wells on the plate. Updates nys october 2010 material number description. Assays are preplated and dried down for a reaction volume of 20 l on 96 well plates and 10 l on 384. The software is compatible with the regulations for electronic records. A multitude of different layouts is available for configuration.
Flexibility of the lightcycler 480 block cycler unit. The lightcycler 96 instrument is a realtime pcr system for rapid cycling up to 96 samples. Roche product information related to the us market. Create your specific gene expression panel with realtime ready assays of your choice on lightcycler 480 multiwell plates 96 or 384, using the realtime ready configurator. Based on a comprehensive improvement of the peltierbased technology incorporated in a silver block, the instrument provides extraordinary welltowell temperature homogeneity and maximized interwell, intercycle reproducibility. Realtime ready custom qpcr assays can be selected for any human, mouse, or rat targets of your choice. Make sure the correct thermal block cycler 96well for f. Roche lightcycler 96 biotecon diagnostics biotecon. Now researchers who currently run protein thermal shift assays on roches lightcycler 480 instrument, using 96 or 384well throughput, can also run these reactions on the lightcycler 1536 instrument, said rama badugu, phd, application support consultant, roche life science.
Component description system component 1 lightcycler instrument sample carousel for o 1. All pcr products are manufactured from virgin, medical grade, bovinefree polypropylene and certified free from dnase, rnase and human dna. The lightcycler 96 instrument is a realtime pcr instrument for rapid cycling up to 96 samples. Since its introduction in 1997, it is now used in many areas of molecular pathology, including oncology solid tumors. Roche introduces thermal shift assay application for. The new lightcycler 96 system offers highly accurate, reproducible, and fast data generation for researchers working in a wide range of fields such as gene expression and genetic variation research. To determine the minimal amount and volume of cdna needed per reaction, we investigated the amplification of a high and a low expressed gene hprt and pitx2 in a serial 1. If using an external standard curve, you must include at least one of the standard concentrations in the new experiment as a standard reference point for calculation. The lightcycler 480 instrument ii is a rapid, platebased, thermal block cycler with integrated realtime, online detection capabilities. For todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected from a lightcycler system.
An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is. Precise lightcycler 96 instrument thermal homogeneity and cycling speed produce accurate and reproducible results in a very short time. Trichomonas vaginalis is the agent of a highly prevalent sexually transmitted infection sti that can result in vaginitis, urethritis, and preterm birth. May 17, 20 for todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected. Plates are available for all major realtime pcr instruments, and can be designed with or without experimental controls. Mar 31, 2015 now researchers who currently run protein thermal shift assays on roches lightcycler 480 instrument, using 96 or 384well throughput, can also run these reactions on the lightcycler 1536 instrument, said rama badugu, phd, application support consultant, roche life science. Purchase and use of the lightcycler 480 instrument, in conjunction with authorized. Our aim is to develop new and improved drugs, diagnostic tests, and services that offer significant benefits over existing options. Ever since its introduction in 1998, the roche applied science lightcycler system has stood for outstanding performance and maximum flexibility. Compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis, mirna research, and relative quantification of target genes. For more information, please consult chapter d, section 4. The cycler has 4 detection channels, which detect hydrolysis 5nuclease taqman probes either as single color or multiplex samples.
A lightcycler probe system consists of a pair of singlestranded fluorescentlabeled oligonucleotides. When used with the lightcycler 480 system, this kit is ideally suited for hotstart pcr applications. Like most website operators, collects nonpersonallyidentifying information of the sort that web browsers and. Vwr 96well pcr plates for roche lightcycler 480 vwr. Roche introduces thermal shift assay application for lightcycler 1536 instrument. In combination with the lightcycler 480 system and suitable pcr primers, this kit allows very sensitive detection and quantification of defined dna sequences. Available with 96well low profile and 384well formats. Traditional methods of diagnosis, including wet preparation, can be unreliable. I am using lightcycler 96 for absolute quantification. A compact and smart device thats the perfect companion for todays. Updates nys october 2010 material number description material group list price % discount price 05212308190 molecular kit capgctm hiv1 v2. Service manual light cycler franks hospital workshop. Oligo probe 1 is labeled at the 3 end with a donor reporter and oligo probe 2 is labeled at its 5 end with one of a few available acceptor reporters.
Lightcycler 96 realtime pcr system super capabilities are. Roche applied science lightcycler 480 realtime pcr system. Methods of color compensation on the lightcycler instruments fampulsar 650 duplexed assays color compensation is used to subtract fluorescent bleedthrough from a reporter into inappropriate channels outside of its dominant channel. As i read the help pdf file on how to use linreg, i.
The lightcycler 480 ii with led is suitable for detection systems with different probe types such as hybridization probes or hydrolysis 5nuclease taqman probes. Methods of color compensation on the lightcycler instruments. Applications include absolute and relative quantification, melting. Applications include absolute and relative quantification, melting curve analysis, and endpoint genotyping. The lightcycler 480 system unleash the potential of real. The lightcycler 96 system embodies roches expertise over the last decade in developing and. Lightcycler 96 system, including instrument startup and shutdown. Roche molecular biochemicals lightcycler operators manual. Service manual light cycler please note that this is only a. Evaluation of optimized reaction chemistry and cdna amount for the lightcycler 1536 instrument.
Create a new experiment from template templates provide a convenient way to speed up the process of creating an experiment. The technology used for the lightcycler system is licensed from idaho technology, inc. Make sure the correct thermal block cycler 96well for f plate or 384well block format for g plate is in place. Microflex pcr plates feature ultrathin and consistent wall thickness on plate wells, to allow fast and efficient heat transfer from the thermal. The record is updated if the fda identifies a violation and classifies the action as a recall, and it is updated for a final time when the recall is terminated. Realtime ready assays are available as single assays for the selected target or preplated on lightcycler 480 multiwell plates 96. Display an online version of an lightcycler software 4 manual. To determine the minimal amount and volume of cdna needed per reaction, we investigated the amplification of a high and a low expressed gene hprt and. Lightcycler 480 realtime pcr system rapid by nature. While unnecessary for singleplex amplifications, color compensation is critical for multiplexed assays. Discover the new lightcycler 96 realtime pcr system. The lightcycler 96 is a realtime pcr cycler for medium sample throughput.
The lightcycler 480 system unleash the potential of realtime pcr. New software releases and user guides for the lightcycler 96 instrument are avail able in the. Lightcycler technology combines rapidcycle polymerase chain reaction with realtime fluorescent monitoring and melting curve analysis. All assays are functiontested and enable fast and efficient expression profiling of your genes of interest. Roche diagnostics lightcycler 480 resolight dye lightcycler 480 resolight dye. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user. Evaluation of the lightcycler 1536 instrument for high.
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